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LncKdm2b controls self‐renewal of embryonic stem cells via activating expression of transcription factor Zbtb3
Author(s) -
Ye Buqing,
Liu Benyu,
Yang Liuliu,
Zhu Xiaoxiao,
Zhang Dongdong,
Wu Wei,
Zhu Pingping,
Wang Yanying,
Wang Shuo,
Xia Pengyan,
Du Ying,
Meng Shu,
Huang Guanling,
Wu Jiayi,
Chen Runsheng,
Tian Yong,
Fan Zusen
Publication year - 2018
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201797174
Subject(s) - biology , embryonic stem cell , transcription factor , microbiology and biotechnology , stem cell , genetics , gene
Divergent long noncoding RNAs (lncRNAs) represent a major lncRNA biotype in mouse and human genomes. The biological and molecular functions of the divergent lncRNAs remain largely unknown. Here, we show that lncKdm2b , a divergent lncRNA for Kdm2b gene, is conserved among five mammalian species and highly expressed in embryonic stem cells (ESCs) and early embryos. LncKdm2b knockout impairs ESC self‐renewal and causes early embryonic lethality. LncKdm2b can activate Zbtb3 by promoting the assembly and ATPase activity of Snf2‐related CREBBP activator protein (SRCAP) complex in trans . Zbtb3 potentiates the ESC self‐renewal in a Nanog‐dependent manner. Finally, Zbtb3 deficiency impairs the ESC self‐renewal and early embryonic development. Therefore, our findings reveal that lncRNAs may represent an additional layer of the regulation of ESC self‐renewal and early embryogenesis.