Premium
Reorganization of inter‐ chromosomal interactions in the 2q37‐deletion syndrome
Author(s) -
Maass Philipp G,
Weise Anja,
Rittscher Katharina,
Lichtenwald Julia,
Barutcu A Rasim,
Liehr Thomas,
Aydin Atakan,
WefeldNeuenfeld Yvette,
Pölsler Laura,
Tinschert Sigrid,
Rinn John L,
Luft Friedrich C,
Bähring Sylvia
Publication year - 2018
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201696257
Subject(s) - biology , genetics , evolutionary biology
Chromosomes occupy distinct interphase territories in the three‐dimensional nucleus. However, how these chromosome territories are arranged relative to one another is poorly understood. Here, we investigated the inter‐ chromosomal interactions between chromosomes 2q, 12, and 17 in human mesenchymal stem cells ( MSC s) and MSC ‐derived cell types by DNA ‐ FISH . We compared our findings in normal karyotypes with a three‐generation family harboring a 2q37‐deletion syndrome, featuring a heterozygous partial deletion of histone deacetylase 4 ( HDAC 4 ) on chr2q37. In normal karyotypes, we detected stable, recurring arrangements and interactions between the three chromosomal territories with a tissue‐specific interaction bias at certain loci. These inter‐ chromosomal interactions were confirmed by Hi‐C. Interestingly, the disease‐related HDAC 4 deletion resulted in displaced inter‐ chromosomal arrangements and altered interactions between the deletion‐affected chromosome 2 and chromosome 12 and/or 17 in 2q37‐deletion syndrome patients. Our findings provide evidence for a direct link between a structural chromosomal aberration and altered interphase architecture that results in a nuclear configuration, supporting a possible molecular pathogenesis.