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The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression
Author(s) -
Egloff Sylvain,
Vitali Patrice,
Tellier Michael,
Raffel Raoul,
Murphy Shona,
Kiss Tamás
Publication year - 2017
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201695740
Subject(s) - snrnp , small nuclear rna , biology , rna polymerase ii , small nuclear ribonucleoprotein , prp24 , rna , transcription (linguistics) , microbiology and biotechnology , rna splicing , gene expression , non coding rna , genetics , gene , promoter , linguistics , philosophy
The 7 SK small nuclear RNP (sn RNP ), composed of the 7 SK small nuclear RNA (sn RNA ), Me PCE , and Larp7, regulates the mRNA elongation capacity of RNA polymerase II ( RNAPII ) through controlling the nuclear activity of positive transcription elongation factor b (P‐ TEF b). Here, we demonstrate that the human 7 SK sn RNP also functions as a canonical transcription factor that, in collaboration with the little elongation complex ( LEC ) comprising ELL , Ice1, Ice2, and ZC 3H8, promotes transcription of RNAPII ‐specific spliceosomal sn RNA and small nucleolar RNA (sno RNA ) genes. The 7 SK sn RNA specifically associates with a fraction of RNAPII hyperphosphorylated at Ser5 and Ser7, which is a hallmark of RNAPII engaged in sn RNA synthesis. Chromatin immunoprecipitation (Ch IP ) and chromatin isolation by RNA purification (Ch IRP ) experiments revealed enrichments for all components of the 7 SK sn RNP on RNAPII ‐specific sn/sno RNA genes. Depletion of 7 SK sn RNA or Larp7 disrupts LEC integrity, inhibits RNAPII recruitment to RNAPII ‐specific sn/sno RNA genes, and reduces nascent sn RNA and sno RNA synthesis. Thus, through controlling both mRNA elongation and sn/sno RNA synthesis, the 7 SK sn RNP is a key regulator of nuclear RNA production by RNAPII.