Premium
Drosophila Dalmatian combines sororin and shugoshin roles in establishment and protection of cohesion
Author(s) -
Yamada Takashi,
Tahara Eri,
Kanke Mai,
Kuwata Keiko,
Nishiyama Tomoko
Publication year - 2017
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201695607
Subject(s) - biology , cohesin , establishment of sister chromatid cohesion , chromosome segregation , microbiology and biotechnology , mitosis , genetics , centromere , meiosis , chromosome , gene
Sister chromatid cohesion is crucial to ensure chromosome bi‐orientation and equal chromosome segregation. Cohesin removal via mitotic kinases and Wapl has to be prevented in pericentromeric regions in order to protect cohesion until metaphase, but the mechanisms of mitotic cohesion protection remain elusive in Drosophila . Here, we show that dalmatian (Dmt), an ortholog of the vertebrate cohesin‐associated protein sororin, is required for protection of mitotic cohesion in flies. Dmt is essential for cohesion establishment during interphase and is enriched on pericentromeric heterochromatin. Dmt is recruited through direct association with heterochromatin protein‐1 ( HP 1), and this interaction is required for cohesion. During mitosis, Dmt interdependently recruits protein phosphatase 2A ( PP 2A) to pericentromeric regions, and PP 2A binding is required for Dmt to protect cohesion. Intriguingly, Dmt is sufficient to protect cohesion upon heterologous expression in human cells. Our findings of a hybrid system, in which Dmt exerts both sororin‐like establishment functions and shugoshin‐like heterochromatin‐based protection roles, provide clues to the evolutionary modulation of eukaryotic cohesion regulation systems.