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IRES ‐mediated translation of cofilin regulates axonal growth cone extension and turning
Author(s) -
Choi JungHyun,
Wang Wei,
Park Dongkeun,
Kim SungHoon,
Kim KyongTai,
Min KyungTai
Publication year - 2018
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201695266
Subject(s) - growth cone , biology , translation (biology) , microbiology and biotechnology , internal ribosome entry site , eukaryotic translation , cone (formal languages) , messenger rna , axon , genetics , gene , computer science , algorithm
In neuronal development, dynamic rearrangement of actin promotes axonal growth cone extension, and spatiotemporal translation of local mRNA s in response to guidance cues directs axonal growth cone steering, where cofilin plays a critical role. While regulation of cofilin activity is well studied, regulatory mechanism for cofilin mRNA translation in neurons is unknown. In eukaryotic cells, proteins can be synthesized by cap‐dependent or cap‐independent mechanism via internal ribosome entry site ( IRES )‐mediated translation. IRES ‐mediated translation has been reported in various pathophysiological conditions, but its role in normal physiological environment is poorly understood. Here, we report that 5′ UTR of cofilin mRNA contains an IRES element, and cofilin is predominantly translated by IRES ‐mediated mechanism in neurons. Furthermore, we show that IRES ‐mediated translation of cofilin is required for both axon extension and axonal growth cone steering. Our results provide new insights into the function of IRES ‐mediated translation in neuronal development.