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DNA  repair by homologous recombination is under stringent cell cycle control. This includes the last step of the reaction, disentanglement of  DNA  joint molecules ( JM s). Previous work has established that  JM  resolving nucleases are activated specifically at the onset of mitosis. In case of budding yeast Mus81‐Mms4, this cell cycle stage‐specific activation is known to depend on phosphorylation by  CDK  and Cdc5 kinases. Here, we show that a third cell cycle kinase, Cdc7‐Dbf4 ( DDK ), targets Mus81‐Mms4 in conjunction with Cdc5—both kinases bind to as well as phosphorylate Mus81‐Mms4 in an interdependent manner. Moreover,  DDK ‐mediated phosphorylation of Mms4 is strictly required for Mus81 activation in mitosis, establishing  DDK  as a novel regulator of homologous recombination. The scaffold protein Rtt107, which binds the Mus81‐Mms4 complex, interacts with Cdc7 and thereby targets  DDK  and Cdc5 to the complex enabling full Mus81 activation. Therefore, Mus81 activation in mitosis involves at least three cell cycle kinases,  CDK , Cdc5 and  DDK . Furthermore, tethering of the kinases in a stable complex with Mus81 is critical for efficient  JM  resolution.

Dbf4‐dependent kinase and the Rtt107 scaffold promote Mus81‐Mms4 resolvase activation during mitosis