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Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control
Author(s) -
Horvath Beatrix M,
Kourova Hana,
Nagy Szilvia,
Nemeth Edit,
Magyar Zoltan,
Papdi Csaba,
Ahmad Zaki,
SanchezPerez Gabino F,
Perilli Serena,
Blilou Ikram,
PettkóSzandtner Aladár,
Darula Zsuzsanna,
Meszaros Tamas,
Binarova Pavla,
Bogre Laszlo,
Scheres Ben
Publication year - 2017
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201694561
Subject(s) - biology , dna damage , retinoblastoma protein , arabidopsis , dna repair , microbiology and biotechnology , genome , genome instability , genetics , heterochromatin , dna , cell cycle , cell , gene , mutant , chromatin
The rapidly proliferating cells in plant meristems must be protected from genome damage. Here, we show that the regulatory role of the Arabidopsis RETINOBLASTOMA RELATED ( RBR ) in cell proliferation can be separated from a novel function in safeguarding genome integrity. Upon DNA damage, RBR and its binding partner E2 FA are recruited to heterochromatic γH2 AX ‐labelled DNA damage foci in an ATM ‐ and ATR ‐dependent manner. These γH2 AX ‐labelled DNA lesions are more dispersedly occupied by the conserved repair protein, At BRCA 1, which can also co‐localise with RBR foci. RBR and At BRCA 1 physically interact in vitro and in planta . Genetic interaction between the RBR ‐silenced ami RBR and Atbrca1 mutants suggests that RBR and At BRCA 1 may function together in maintaining genome integrity. Together with E2 FA , RBR is directly involved in the transcriptional DNA damage response as well as in the cell death pathway that is independent of SOG 1, the plant functional analogue of p53. Thus, plant homologs and analogues of major mammalian tumour suppressor proteins form a regulatory network that coordinates cell proliferation with cell and genome integrity.