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Microtubule‐binding protein doublecortin‐like kinase 1 (DCLK1) guides kinesin‐3‐mediated cargo transport to dendrites
Author(s) -
Lipka Joanna,
Kapitein Lukas C,
Jaworski Jacek,
Hoogenraad Casper C
Publication year - 2016
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201592929
Subject(s) - kinesin , microtubule , biology , motor protein , microbiology and biotechnology , doublecortin , molecular motor , vesicular transport protein , microtubule associated protein , transport protein , axoplasmic transport , vesicle , hippocampal formation , neuroscience , biochemistry , dentate gyrus , membrane
In neurons, the polarized distribution of vesicles and other cellular materials is established through molecular motors that steer selective transport between axons and dendrites. It is currently unclear whether interactions between kinesin motors and microtubule‐binding proteins can steer polarized transport. By screening all 45 kinesin family members, we systematically addressed which kinesin motors can translocate cargo in living cells and drive polarized transport in hippocampal neurons. While the majority of kinesin motors transport cargo selectively into axons, we identified five members of the kinesin‐3 ( KIF 1) and kinesin‐4 ( KIF 21) subfamily that can also target dendrites. We found that microtubule‐binding protein doublecortin‐like kinase 1 ( DCLK 1) labels a subset of dendritic microtubules and is required for KIF 1‐dependent dense‐core vesicles ( DCV s) trafficking into dendrites and dendrite development. Our study demonstrates that microtubule‐binding proteins can provide local signals for specific kinesin motors to drive polarized cargo transport.