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Transcriptional repression by MYB 3R proteins regulates plant organ growth
Author(s) -
Kobayashi Kosuke,
Suzuki Toshiya,
Iwata Eriko,
Nakamichi Norihito,
Suzuki Takamasa,
Chen Poyu,
Ohtani Misato,
Ishida Takashi,
Hosoya Hanako,
Müller Sabine,
Leviczky Tünde,
PettkóSzandtner Aladár,
Darula Zsuzsanna,
Iwamoto Akitoshi,
Nomoto Mika,
Tada Yasuomi,
Higashiyama Tetsuya,
Demura Taku,
Doonan John H,
Hauser MarieTheres,
Sugimoto Keiko,
Umeda Masaaki,
Magyar Zoltán,
Bögre László,
Ito Masaki
Publication year - 2015
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201490899
Subject(s) - library science , biological sciences , chinese academy of sciences , graduate students , biology , political science , sociology , china , microbiology and biotechnology , computer science , law , pedagogy
In multicellular organisms, temporal and spatial regulation of cell proliferation is central for generating organs with defined sizes and morphologies. For establishing and maintaining the post‐mitotic quiescent state during cell differentiation, it is important to repress genes with mitotic functions. We found that three of the Arabidopsis MYB 3R transcription factors synergistically maintain G2/M‐specific genes repressed in post‐mitotic cells and restrict the time window of mitotic gene expression in proliferating cells. The combined mutants of the three repressor‐type MYB 3R genes displayed long roots, enlarged leaves, embryos, and seeds. Genome‐wide chromatin immunoprecipitation revealed that MYB 3R3 binds to the promoters of G2/M‐specific genes and to E2F target genes. MYB 3R3 associates with the repressor‐type E2F, E2 FC , and the RETINOBLASTOMA RELATED proteins. In contrast, the activator MYB 3R4 was in complex with E2 FB in proliferating cells. With mass spectrometry and pairwise interaction assays, we identified some of the other conserved components of the multiprotein complexes, known as DREAM / dREAM in human and flies. In plants, these repressor complexes are important for periodic expression during cell cycle and to establish a post‐mitotic quiescent state determining organ size.