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A NAD ‐dependent glutamate dehydrogenase coordinates metabolism with cell division in Caulobacter crescentus
Author(s) -
Beaufay François,
Coppine Jérôme,
Mayard Aurélie,
Laloux Géraldine,
De Bolle Xavier,
Hallez Régis
Publication year - 2015
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201490730
Subject(s) - caulobacter crescentus , biology , glutamate dehydrogenase , nad+ kinase , cell division , biochemistry , dehydrogenase , microbiology and biotechnology , metabolism , enzyme , glutamate receptor , cell , cell cycle , receptor
Coupling cell cycle with nutrient availability is a crucial process for all living cells. But how bacteria control cell division according to metabolic supplies remains poorly understood. Here, we describe a molecular mechanism that coordinates central metabolism with cell division in the α‐proteobacterium Caulobacter crescentus . This mechanism involves the NAD ‐dependent glutamate dehydrogenase GdhZ and the oxidoreductase‐like KidO. While enzymatically active GdhZ directly interferes with FtsZ polymerization by stimulating its GTP ase activity, KidO bound to NADH destabilizes lateral interactions between FtsZ protofilaments. Both GdhZ and KidO share the same regulatory network to concomitantly stimulate the rapid disassembly of the Z‐ring, necessary for the subsequent release of progeny cells. Thus, this mechanism illustrates how proteins initially dedicated to metabolism coordinate cell cycle progression with nutrient availability.