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Cross talk between ABC transporter m RNA s via a target m RNA ‐derived sponge of the G cv B small RNA
Author(s) -
Miyakoshi Masatoshi,
Chao Yanjie,
Vogel Jörg
Publication year - 2015
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201490546
Subject(s) - rnase p , rna , messenger rna , regulon , biology , microbiology and biotechnology , biochemistry , gene , gene expression
There is an expanding list of examples by which one mRNA can posttranscriptionally influence the expression of others. This can involve RNA sponges that sequester regulatory RNA s of mRNA s in the same regulon, but the underlying molecular mechanism of such mRNA cross talk remains little understood. Here, we report sponge‐mediated mRNA cross talk in the posttranscriptional network of GcvB, a conserved Hfq‐dependent small RNA with one of the largest regulons known in bacteria. We show that mRNA decay from the glt IJKL locus encoding an amino acid ABC transporter generates a stable fragment (SroC) that base‐pairs with GcvB. This interaction triggers the degradation of GcvB by RN ase E, alleviating the GcvB‐mediated mRNA repression of other amino acid‐related transport and metabolic genes. Intriguingly, since the glt IJKL mRNA itself is a target of GcvB, the SroC sponge seems to enable both an internal feed‐forward loop to activate its parental mRNA in cis and activation of many trans ‐encoded mRNA s in the same pathway. Disabling this mRNA cross talk affects bacterial growth when peptides are the sole carbon and nitrogen sources.