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RBM 14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity
Author(s) -
Shiratsuchi Gen,
Takaoka Katsuyoshi,
Ashikawa Tomoko,
Hamada Hiroshi,
Kitagawa Daiju
Publication year - 2014
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201488979
Subject(s) - biology , microbiology and biotechnology , mitosis , spindle apparatus , cell cycle protein , spindle pole body , cell division , biochemistry , cell cycle , gene , cell
Formation of a new centriole adjacent to a pre‐existing centriole occurs only once per cell cycle. Despite being crucial for genome integrity, the mechanisms controlling centriole biogenesis remain elusive. Here, we identify RBM 14 as a novel suppressor of assembly of centriolar protein complexes. Depletion of RBM 14 in human cells induces ectopic formation of centriolar protein complexes through function of the STIL / CPAP complex. Intriguingly, the formation of such structures seems not to require the cartwheel structure that normally acts as a scaffold for centriole formation, whereas they can retain pericentriolar material and microtubule nucleation activity. Moreover, we find that, upon RBM 14 depletion, a part of the ectopic centriolar protein complexes in turn assemble into structures more akin to centrioles, presumably by incorporating Hs SAS ‐6, a cartwheel component, and cause multipolar spindle formation. We further demonstrate that such structures assemble in the cytoplasm even in the presence of pre‐existing centrioles. This study sheds light on the possibility that ectopic formation of aberrant structures related to centrioles may contribute to genome instability and tumorigenesis.