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Identification of C dk targets that control cytokinesis
Author(s) -
Kuilman Thomas,
Maiolica Alessio,
Godfrey Molly,
Scheidel Noémie,
Aebersold Ruedi,
Uhlmann Frank
Publication year - 2014
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201488958
Subject(s) - cytokinesis , biology , identification (biology) , computational biology , microbiology and biotechnology , genetics , cell division , cell , botany
The final event of the eukaryotic cell cycle is cytokinesis, when two new daughter cells are born. How the timing and execution of cytokinesis is controlled is poorly understood. Here, we show that downregulation of cyclin‐dependent kinase ( C dk) activity, together with upregulation of its counteracting phosphatase C dc14, controls each of the sequential steps of cytokinesis, including furrow ingression, membrane resolution and cell separation in budding yeast. We use phosphoproteome analysis of mitotic exit to identify C dk targets that are dephosphorylated at the time of cytokinesis. We then apply a new and widely applicable tool to generate conditionally phosphorylated proteins to identify those whose dephosphorylation is required for cytokinesis. This approach identifies A ip1, E de1 and I nn1 as cytokinetic regulators. Our results suggest that cytokinesis is coordinately controlled by the master cell cycle regulator C dk together with its counteracting phosphatase and that it is executed by concerted dephosphorylation of C dk targets involved in several cell biological processes.