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NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane
Author(s) -
Kotak Sachin,
Busso Coralie,
Gönczy Pierre
Publication year - 2014
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201488147
Subject(s) - biology , mitosis , microbiology and biotechnology , spindle apparatus , membrane , cell division , genetics , cell
The positioning and the elongation of the mitotic spindle must be carefully regulated. In human cells, the evolutionary conserved proteins LGN /Gα i1‐3 anchor the coiled‐coil protein Nu MA and dynein to the cell cortex during metaphase, thus ensuring proper spindle positioning. The mechanisms governing cortical localization of Nu MA and dynein during anaphase remain more elusive. Here, we report that LGN /Gα i1‐3 are dispensable for Nu MA ‐dependent cortical dynein enrichment during anaphase. We further establish that Nu MA is excluded from the equatorial region of the cell cortex in a manner that depends on the centralspindlin components CYK 4 and MKLP 1. Importantly, we reveal that Nu MA can directly associate with PtdInsP ( PIP ) and PtdInsP 2 ( PIP 2 ) phosphoinositides in vitro . Furthermore, chemical or enzymatic depletion of PIP / PIP 2 prevents Nu MA cortical localization during mitosis, and conversely, increasing PIP 2 levels augments mitotic cortical Nu MA . Overall, our study uncovers a novel function for plasma membrane phospholipids in governing cortical Nu MA distribution and thus the proper execution of mitosis.