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Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis
Author(s) -
Özlü Nurhan,
Qureshi Mohammad H,
Toyoda Yusuke,
Renard Bernhard Y,
Mollaoglu Gürkan,
Özkan Nazlı E,
Bulbul Selda,
Poser Ina,
Timm Wiebke,
Hyman Anthony A,
Mitchison Timothy J,
Steen Judith A
Publication year - 2014
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201385162
Subject(s) - biology , interphase , mitosis , proteome , cancer , microbiology and biotechnology , cell cycle , computational biology , cell , genetics
Abstract The cell surface is the cellular compartment responsible for communication with the environment. The interior of mammalian cells undergoes dramatic reorganization when cells enter mitosis. These changes are triggered by activation of the CDK 1 kinase and have been studied extensively. In contrast, very little is known of the cell surface changes during cell division. We undertook a quantitative proteomic comparison of cell surface‐exposed proteins in human cancer cells that were tightly synchronized in mitosis or interphase. Six hundred and twenty‐eight surface and surface‐associated proteins in HeLa cells were identified; of these, 27 were significantly enriched at the cell surface in mitosis and 37 in interphase. Using imaging techniques, we confirmed the mitosis‐selective cell surface localization of protocadherin PCDH 7, a member of a family with anti‐adhesive roles in embryos. We show that PCDH 7 is required for development of full mitotic rounding pressure at the onset of mitosis. Our analysis provided basic information on how cell cycle progression affects the cell surface. It also provides potential pharmacodynamic biomarkers for anti‐mitotic cancer chemotherapy.