Open AccessMacro photography with Lighsheet Illumination Enables Whole Expanded Brain Imaging with Single-cell Resolution
Author(s) -
Chia-Ming Lee,
Xuejiao Tian,
Chieh Tsao,
Peilin Chen,
Tzyy-Nan Huang,
YiPing Hsueh,
Bi-Chang Chen
Publication year - 2021
Publication title -
discoveries
Language(s) - English
Resource type - Journals
ISSN - 2359-7232
DOI - 10.15190/d.2021.12
Subject(s) - magnification , lens (geology) , resolution (logic) , photography , optics , microscopy , field of view , visualization , materials science , macro , camera lens , microscope , image resolution , computer science , computer vision , artificial intelligence , physics , art , visual arts , programming language
Macro photography allows direct visualization of the enlarged whole mouse brain by a combination of lightsheet illumination and expansion microscopy with single-cell resolution. Taking advantage of the long working distance of a camera lens, we imaged a 3.7 cm thick, transparent, fluorescently-labeled expanded brain. In order to improve 3D sectioning capability, we used lightsheet excitation confined as the depth of field of the camera lens. Using 4x sample expansion and 5x optical magnification, macro photography enables imaging of expanded whole mouse brain with an effective resolution of 300 nm, which provides the subcellular structural information at the organ level.