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open-access-imgOpen AccessThe Impact of CO2 Laser Treatment and Acidulated Phosphate Fluoride on Enamel Demineralization and Biofilm Formation
Author(s)
Ana Maria de Araújo Loiola,
Carolina Patrícia Aires,
Fabiana Almeida Curylofo-Zotti,
António Rodrigues,
Aline Evangelista Souza-Gabriel,
Silmara Aparecida Milori Corona
Publication year2019
Publication title
journal of lasers in medical sciences
Resource typeJournals
PublisherMaad Rayan Publishing Company
Introduction: This study evaluated the impact of CO2 laser treatment and acidulated phosphate fluoride (APF) on enamel demineralization and biofilm formation, using in vitro and in situ designs. Methods: Demineralized enamel slabs were distributed among 8 groups: placebo, placebo + continuous CO2 laser, placebo + repeated CO2 laser, placebo + ultrapulsed CO2 laser, 1.23% APF, APF + continuous CO2 laser, APF + repeated CO2 laser and APF + ultrapulsed CO2 laser. In the in vitro study, 15 enamel slabs from each group were subjected to a pH-cycling regimen for 14 days. In the cross over in situ design, 11 volunteers wore palatal appliances with demineralized enamel slabs for 2 periods of 14 days each. Drops of sucrose solution were dripped onto enamel slabs 8×/day. Biofilms formed on slabs were collected and the colony-forming units (CFU) of Streptococcus mutans and Lactobacillus were determined. Results: For both in vitro and in situ studies, there was no significant difference between treatments (P>0.05). However, all treatments increased microhardness of demineralized enamel (P 0.05) or Lactobacillus (P>0.05) counts between groups. Conclusion: The results suggest that APF gel combined with the CO2 laser, regardless of the pulse emission mode used, was effective in controlling enamel demineralization, but none of the tested treatments was able to prevent bacterial colonization.
Subject(s)alternative medicine , bacteria , biofilm , biology , chemistry , demineralization , dentistry , enamel paint , fluoride , genetics , inorganic chemistry , medicine , pathology , placebo , streptococcus mutans
Language(s)English
SCImago Journal Rank0.443
H-Index21
ISSN2008-9783
DOI10.15171/jlms.2019.32

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