Open AccessSampling, Maintenance and Pathotype Identification of Synchytrium Endobioticum (Schilb.) Perc
Author(s) -
Jarosław Przetakiewicz
Publication year - 2017
Publication title -
plant breeding and seed science
Language(s) - English
Resource type - Journals
eISSN - 2083-599X
pISSN - 1429-3862
DOI - 10.1515/plass-2017-0018
Subject(s) - biology , sporangium , cultivar , horticulture , identification (biology) , botany , spore
Plant Breeding and Acclimatization Institute - National Research Institute (IHAR-PIB) is responsible for pathotype identification of Synchytrium endobioticum isolates collecting from Poland. Pathogenicity tests are carried out using the Glynne-Lemmerzahl method, according to EPPO Standard PM 7/28. Pathotypes are defined based on their reaction to a range of well-characterized differential cultivars of potato. Assessment of one isolate of the fungus requires more than 20 differential cultivars. All pathotypes of S. endobioticum from Polish reference collection are multiplied and maintained (fresh warts) on tubers of extremely susceptible cultivars continuously. For all references pathotypes and isolates of S. endobioticum, the compost with winter sporangia are prepared for long-term of maintenance.