Open AccessSilica Monolithic Capillary Columns for Hilic Separations
Author(s) -
Dana Moravcová,
Josef Planeta
Publication year - 2018
Publication title -
hungarian journal of industry and chemistry/hungarian journal of industrial chemistry
Language(s) - English
Resource type - Journals
eISSN - 2450-5102
pISSN - 0133-0276
DOI - 10.1515/hjic-2018-0009
Subject(s) - chemistry , ammonium hydroxide , polyethylene glycol , monolith , selectivity , chromatography , hydrophilic interaction chromatography , capillary electrochromatography , capillary action , hydrolysis , silica gel , peg ratio , ammonium acetate , silicon dioxide , stationary phase , chemical engineering , high performance liquid chromatography , organic chemistry , materials science , catalysis , finance , economics , composite material , engineering
The monolithic capillary columns (0.1 mm x 150 mm) prepared by the acidic hydrolysis of tetramethoxysilane (TMOS) in the presence of polyethylene glycol (PEG) and urea were modified by zwitterionic stationary phases and evaluated under HILIC separation conditions by employing a mixture containing nucleosides and nucleotides. The polymeric layer of zwitterions did not affect the high separation efficiency of the original silica monolith. The prepared zwitterionic columns exhibited high separation efficiencies in a range 61,000-289,000 theoretical plates/m for a 2- methacryloyloxyethyl phosphorylcholine-based stationary phase and in a range 59,000-135,000 theoretical plates/m for a [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide-based stationary phase under optimal separation conditions. The grafted layer of zwitterions on the silica monolithic surface also significantly improved the separation selectivity to compounds of interest