Silica Monolithic Capillary Columns for Hilic Separations | Zendy

Dana Moravcová | Zendy; Josef Planeta | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Silica Monolithic Capillary Columns for Hilic Separations

Author(s) -

Dana Moravcová,

Josef Planeta

Publication year - 2018

Publication title -

hungarian journal of industry and chemistry

Language(s) - English

Resource type - Journals

eISSN - 2450-5102

pISSN - 0133-0276

DOI - 10.1515/hjic-2018-0009

Subject(s) - monolith , chemistry , ammonium hydroxide , polyethylene glycol , capillary electrochromatography , selectivity , chromatography , hydrophilic interaction chromatography , capillary action , peg ratio , monolithic hplc column , hydrolysis , ammonium acetate , stationary phase , high performance liquid chromatography , organic chemistry , materials science , catalysis , finance , economics , composite material

The monolithic capillary columns (0.1 mm x 150 mm) prepared by the acidic hydrolysis of tetramethoxysilane (TMOS) in the presence of polyethylene glycol (PEG) and urea were modified by zwitterionic stationary phases and evaluated under HILIC separation conditions by employing a mixture containing nucleosides and nucleotides. The polymeric layer of zwitterions did not affect the high separation efficiency of the original silica monolith. The prepared zwitterionic columns exhibited high separation efficiencies in a range 61,000-289,000 theoretical plates/m for a 2- methacryloyloxyethyl phosphorylcholine-based stationary phase and in a range 59,000-135,000 theoretical plates/m for a [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide-based stationary phase under optimal separation conditions. The grafted layer of zwitterions on the silica monolithic surface also significantly improved the separation selectivity to compounds of interest

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research