Open AccessLC-MS/MS Determination of Tropane Alkaloids in Maize Crop
Author(s) -
Gorica Vuković,
Vojislava Bursić,
Tijana Stojanović,
Aleksandra Petrović,
Sonja Gvozdenac,
Mira Starović,
Slobodan Kuzmanović,
Goran Aleksić
Publication year - 2018
Publication title -
contemporary agriculture
Language(s) - English
Resource type - Journals
ISSN - 2466-4774
DOI - 10.1515/contagri-2018-0032
Subject(s) - tropane , chemistry , chromatography , atropine , sodium , acetic acid , analyte , hyoscyamine , scopolamine , methanol , extraction (chemistry) , datura stramonium , pharmacology , biochemistry , stereochemistry , botany , solanaceae , organic chemistry , biology , gene , endocrinology
Summary The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.