Open AccessA comparative study of phenolic compound antioxidant activity by the polarography method, using microsomal lipid peroxidation in vitro
Author(s) -
Anatolii Gordiienko,
Mykola Blazheyevskyi,
Ivan Iurchenko
Publication year - 2018
Publication title -
current issues in pharmacy and medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.134
H-Index - 9
eISSN - 2300-6676
pISSN - 2084-980X
DOI - 10.1515/cipms-2018-0034
Subject(s) - myricetin , antioxidant , lipid peroxidation , flavonols , chemistry , mangiferin , microsome , catechin , quercetin , kaempferol , polyphenol , biochemistry , autoxidation , organic chemistry , in vitro , chromatography
For comparative purposes, a quantitative estimation of antioxidant activity of phenolic compounds of different classes was conducted by way of the polarography method, via the ADP-Fe 2+ model of the induced ascorbate-dependent lipid peroxidation of rat liver micro-somes within an in-vitro system. As a result, it was recognized that the antioxidant properties of phenolic compounds depend on the nature and chemical structure of several substances. In respect of such activity, leaders in the classes of investigated polyphenolic compounds are: Propyl gallate = Gallotannin (Phenolcarboxylic acids and their derivatives) > Quercetin = Myricetin (Flavonols) > Luteolin (Flavo n) = Mangiferin (Xanthones) > Kaempferol (Flavonols) = Catechin (Flavans). Thus, the assessment of the inhibition ability of the lipid peroxidation of microsomes by phenolic compounds can be used as an accessible test for the preliminary quantitative estimation of their antioxidant properties.