Open AccessCharacterization of perfusion decellularized whole animal body, isolated organs, and multi‐organ systems for tissue engineering applications
Author(s) -
Taylor Doris A.,
Kren Stefan M.,
Rhett Katrina,
Robertson Matthew J.,
Morrissey Jacquelynn,
Rodriguez Osman E.,
Virk Hassan,
ChaconAlberty Lourdes,
Curty da Costa Ernesto,
Mesquita Fernanda C. P.,
Sampaio Luiz C.,
HochmanMendez Camila
Publication year - 2021
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.14817
Subject(s) - decellularization , perfusion , extracellular matrix , tissue engineering , biomedical engineering , kidney , lung , pathology , medicine , anatomy , scaffold , elastin , organ system , biology , microbiology and biotechnology , disease
To expand the application of perfusion decellularization beyond isolated single organs, we used the native vasculature of adult and neonatal rats to systemically decellularize the organs of a whole animal in situ. Acellular scaffolds were generated from kidney, liver, lower limb, heart‐lung system, and a whole animal body, demonstrating that perfusion decellularization technology is applicable to any perfusable tissue, independent of age. Biochemical and histological analyses demonstrated that organs and organ systems (heart‐lung pair and lower limb) were successfully decellularized, retaining their extracellular matrix (ECM) structure and organ‐specific composition, as evidenced by differences in organ‐specific scaffold stiffness. Altogether, we demonstrated that organs, organ systems and whole animal bodies can be perfusion decellularized while retaining ECM components and biomechanics.