Open AccessThe order of concurrent training affects mTOR signaling but not mitochondrial biogenesis in mouse skeletal muscle
Author(s) -
Shirai Takanaga,
Aoki Yuki,
Takeda Kohei,
Takemasa Tohru
Publication year - 2020
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.14411
Subject(s) - mitochondrial biogenesis , endurance training , skeletal muscle , ribosomal protein s6 , muscle hypertrophy , ribosome biogenesis , p70 s6 kinase 1 , phosphorylation , biology , pi3k/akt/mtor pathway , signal transduction , mitochondrion , medicine , microbiology and biotechnology , endocrinology , gene , biochemistry , ribosome , rna
Concurrent training involves a combination of two different modes of training. In this study, we conducted an experiment by combining resistance and endurance training. The purpose of this study was to investigate the influence of the order of concurrent training on signal molecules in skeletal muscle. The phosphorylation levels of p70 S6 kinase, S6 ribosomal protein, and 4E‐binding protein 1, which are related to hypertrophy signaling, increased significantly in the resistance–endurance order group as compared with in control group not the endurance–resistance order group. The gene expressions related to metabolism were not changed by the order of concurrent training. The mitochondrial respiratory chain complex was evaluated by western blot. Although both groups of concurrent training showed a significant increase in MTCO1, UQCRC2, and ATP5A protein levels, we could not detect a difference based on the order of concurrent training. In conclusion, a concurrent training approach involving resistance training before endurance training on the same day is an effective way to activate both mTOR signaling and mitochondria biogenesis.