Open AccessGhrelin stimulates fatty acid oxidation and inhibits lipolysis in isolated muscle from male rats
Author(s) -
Kraft Emily N.,
Cervone Daniel T.,
Dyck David J.
Publication year - 2019
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.14028
Subject(s) - lipolysis , ghrelin , endocrinology , medicine , beta oxidation , acetyl coa carboxylase , fatty acid , chemistry , ampk , lipid oxidation , fatty acid metabolism , lipid metabolism , skeletal muscle , adipose tissue , phosphorylation , biology , metabolism , pyruvate carboxylase , biochemistry , hormone , enzyme , protein kinase a , antioxidant
Ghrelin is classically known as a central appetite‐stimulating hormone but has recently been recognized to have a significant role in peripheral tissue energy metabolism. However, the direct effects of ghrelin on skeletal muscle, a major site for glucose and lipid disposal, remain understudied. We found that the two major ghrelin isoforms, acylated and unacylated ghrelin, were able to significantly increase skeletal muscle fatty acid oxidation (~20%) while incorporation of fatty acids into major lipid pools remained unchanged. The increase in fatty acid oxidation was accompanied by increases in acetyl‐CoA carboxylase phosphorylation, a downstream target of AMPK . Ghrelin isoforms had no independent effect on lipolysis under unstimulated conditions, but nearly completely abolished epinephrine‐stimulated lipolysis. This effect was generally, but not consistently related to a blunting in the phosphorylation of HSL activation sites, Ser660 and 563. Taken together, these findings suggest that ghrelin isoforms have a direct, acute effect on fatty acid oxidation and lipolysis.