Open AccessEffects of HIV ‐1 gp120 and tat on endothelial cell sensescence and senescence‐associated micro RNA s
Author(s) -
Hijmans Jamie G.,
Stockleman Kelly,
Reiakvam Whitney,
Levy Ma'ayan V.,
Brewster Lillian M.,
Bammert Tyler D.,
Greiner Jared J.,
Connick Elizabeth,
DeSouza Christopher A.
Publication year - 2018
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.13647
Subject(s) - physiology , biology
The aim of this study was to determine, in vitro, the effects of X4 and R5 HIV ‐1 gp120 and Tat on: (1) endothelial cell senescence and (2) endothelial cell micro RNA (miR) expression. Endothelial cells were treated with media without and with: R5 gp120 (100 ng/mL), X4 gp120 (100 ng/mL), or Tat (500 ng/mL) for 24 h and stained for senescence‐associated β ‐galactosidase ( SA ‐ β ‐gal). Cell expression of miR‐34a, miR‐217, and miR‐146a was determined by RT ‐ PCR . X4 and R5 gp120 and Tat significantly increased (~100%) cellular senescence versus control. X4 gp120 significantly increased cell expression of miR‐34a (1.60 ± 0.04 fold) and miR‐217 (1.52 ± 0.18), but not miR‐146a (1.25 ± 0.32). R5 gp120 significantly increased miR‐34a (1.23 ± 0.07) and decreased miR‐146a (0.56 ± 0.07). Tat significantly increased miR‐34a (1.49 ± 0.16) and decreased miR‐146a (0.55 ± 0.23). R5 and Tat had no effect on miR‐217 (1.05 ± 0.13 and 1.06 ± 0.24; respectively). HIV ‐1 gp120 (X4 and R5) and Tat promote endothelial cell senescence and dysregulation of senescence‐associated miRs.