Open AccessChloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP‐activated whole‐cell chloride currents in human bronchial epithelial cells
Author(s) -
Liu Bo,
Billington Charlotte K.,
Henry Amanda P.,
Bhaker Sangita K.,
Kheirallah Alexander K.,
Swan Caroline,
Hall Ian P.
Publication year - 2018
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.13508
Subject(s) - chloride channel , intracellular , chloride , microbiology and biotechnology , ion channel , cell , chemistry , cell type , biophysics , biology , biochemistry , receptor , organic chemistry
Chloride channels are known to play critical physiological roles in many cell types. Here, we describe the expression of anion channels using RNA Seq in primary cultures of human bronchial epithelial cells (hBECs). Chloride intracellular channel (CLIC) family members were the most abundant chloride channel transcripts, and CLIC1 showed the highest level of expression. In addition, we characterize the chloride currents in hBECs and determine how inhibition of CLIC1 via pharmacological and molecular approaches impacts these. We demonstrate that CLIC1 is able to modulate cyclic AMP‐induced chloride currents and suggest that CLIC1 modulation could be important for chloride homeostasis in this cell type.