MiR‐335 overexpression impairs insulin secretion through defective priming of insulin vesicles

Micro RNA s contribute to the maintenance of optimal cellular functions by fine‐tuning protein expression levels. In the pancreatic β ‐cells, imbalances in the exocytotic machinery components lead to impaired insulin secretion and type 2 diabetes (T2D). We hypothesize that dysregulated mi RNA expression exacerbates β ‐cell dysfunction, and have earlier shown that islets from the diabetic GK ‐rat model have increased expression of mi RNA s, including miR‐335‐5p (miR‐335). Here, we aim to determine the specific role of miR‐335 during development of T2D, and the influence of this mi RNA on glucose‐stimulated insulin secretion and Ca 2+ ‐dependent exocytosis. We found that the expression of miR‐335 negatively correlated with secretion index in human islets of individuals with prediabetes. Overexpression of miR‐335 in human EndoC‐ β H1 and in rat INS ‐1 832/13 cells ( OE 335) resulted in decreased glucose‐stimulated insulin secretion, and OE 335 cells showed concomitant reduction in three exocytotic proteins: SNAP 25, Syntaxin‐binding protein 1 ( STXBP 1), and synaptotagmin 11 ( SYT 11). Single‐cell capacitance measurements, complemented with TIRF microscopy of the granule marker NPY ‐ mEGFP demonstrated a significant reduction in exocytosis in OE 335 cells. The reduction was not associated with defective docking or decreased Ca 2+ current. More likely, it is a direct consequence of impaired priming of already docked granules. Earlier reports have proposed reduced granular priming as the cause of reduced first‐phase insulin secretion during prediabetes. Here, we show a specific role of miR‐335 in regulating insulin secretion during this transition period. Moreover, we can conclude that miR‐335 has the capacity to modulate insulin secretion and Ca 2+ ‐dependent exocytosis through effects on granular priming.