Open AccessAssessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography
Author(s) -
Beikoghli Kalkhoran Siavash,
Hall Andrew R.,
White Ian J.,
Cooper Jackie,
Fan Qiao,
Ong SangBing,
HernándezReséndiz Sauri,
CabreraFuentes Hector,
Chinda Kroekkiat,
Chakraborty Bibhas,
Dorn Gerald W.,
Yellon Derek M.,
Hausenloy Derek J.
Publication year - 2017
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.13437
Subject(s) - mfn2 , mitochondrion , electron microscope , electron tomography , endoplasmic reticulum , chemistry , biology , microbiology and biotechnology , biophysics , mitochondrial fusion , biochemistry , mitochondrial dna , physics , scanning transmission electron microscopy , optics , gene
The effects of mitofusin 2 ( MFN 2) deficiency, on mitochondrial morphology and the mitochondria–junctional sarcoplasmic reticulum ( jSR ) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN 2‐deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria– jSR contacts, and an increase in the distance between mitochondria and jSR , when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria– jSR complex in the adult heart.