Open AccessMyocardial apoptosis and mesenchymal stem cells with acute exercise
Author(s) -
Arisi Maria F.,
Chirico Erica N.,
Sebeny Roxanne,
Muthukumaran Geetha,
Mu Anbin,
De Jonghe Bart C.,
Margulies Kenneth B.,
Libonati Joseph R.
Publication year - 2017
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.13297
Subject(s) - mesenchymal stem cell , medicine , aerobic exercise , apoptosis , endocrinology , inflammation , paracrine signalling , biology , pathology , biochemistry , receptor
Abstract Aerobic exercise confers many health benefits. However, numerous reports have shown that acute aerobic exercise can injure the heart. We tested the general hypothesis that acute moderate‐intensity exercise in rodents induces cardiomyocyte damage and stimulates mesenchymal stem cells ( MSC s) to increase paracrine‐mediated protective effects on cardiomyocytes. A single session of treadmill running (13 m/min, 0% grade, for 45 min) in untrained C57 BL /6 male mice ( n = 18) increased cleaved poly ADP ‐ribose polymerase ( PARP ), a marker of apoptosis, in the myocardium 24 h postexercise. Microarray analysis of mouse myocardium identified 11 relevant apoptotic genes and several shifts in matrix remodeling transcripts over the postexercise window. Postexercise cardiomyocyte death was recapitulated in neonatal rat cardiomyocytes ( NRCM s) by culturing cells in 2% plasma harvested from exercised rats. The increased cell death observed in exercise‐treated NRCM s was attenuated by β ‐adrenergic blockade, but not antioxidant treatment. MSC survival, proliferation, and chemotaxis showed no significant differences between sedentary and exercise plasma conditions, despite increased IL ‐6, TNF ‐ α , IL ‐1 β , and IFN ‐ γ secretions from MSC s treated with exercise plasma. NRCM survival was increased nearly 500% when cocultured with MSC s, but this effect was not altered under exercise plasma culture conditions. Our results suggest acute moderate‐intensity aerobic treadmill running in exercise‐naïve rodents induces temporal cardiomyocyte death due to plasma‐borne factors, namely, catecholaminergic stress. Even though exercise conditions prompt an inflammatory response in MSC s, the exercise milieu does not alter the MSC ‐protective phenotype on cardiomyocytes.