Open AccessParadoxical effect of IKKβ inhibition on the expression of E3 ubiquitin ligases and unloading‐induced skeletal muscle atrophy
Author(s) -
Belova Svetlana P.,
Shenkman Boris S.,
Kostrominova Tatiana Y.,
Nemirovskaya Tatiana L.
Publication year - 2017
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.13291
Subject(s) - ubiquitin , muscle atrophy , atrophy , skeletal muscle , endocrinology , in vivo , medicine , ubiquitin ligase , hindlimb , downregulation and upregulation , iκb kinase , p50 , chemistry , biology , nf κb , biochemistry , inflammation , microbiology and biotechnology , gene , transcription factor
We tested whether NF ‐ κ B pathway is indispensable for the increase in expression of E3‐ligases and unloading‐induced muscle atrophy using IKK β inhibitor IMD ‐0354. Three groups of rats were used: nontreated control (C), 3 days of unloading/hindlimb suspension with ( HS + IMD ) or without ( HS ) IMD ‐0354. Levels of I κ B α were higher in HS + IMD (1.16‐fold) and lower in HS (0.82‐fold) when compared with C group. IMD ‐0354 treatment during unloading: had no effect on loss of muscle mass; increased mRNA levels of Mu RF 1 and MAF bx; increased level s of pF oxO3; and had no effect on levels of Bcl‐3, p105, and p50 proteins. Our study for the first time showed that inhibiting IKK β in vivo during 3‐day unloading failed to diminish expression of ubiquitin ligases and prevent muscle atrophy.