Open AccessAn advanced glycation endproduct ( AGE )‐rich diet promotes accumulation of AGE s in Achilles tendon
Author(s) -
Skovgaard Dorthe,
Svensson Rene B.,
Scheijen Jean,
Eliasson Pernilla,
Mogensen Pernille,
Hag Anne Mette F.,
Kjær Michael,
Schalkwijk Casper G.,
Schjerling Peter,
Magnusson Stig P.,
Couppé Christian
Publication year - 2017
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.13215
Subject(s) - pentosidine , achilles tendon , tendon , glycation , chemistry , medicine , endocrinology , anatomy , diabetes mellitus
Advanced Glycation Endproducts ( AGE s) accumulate in long‐lived tissue proteins like collagen in bone and tendon causing modification of the biomechanical properties. This has been hypothesized to raise the risk of orthopedic injury such as bone fractures and tendon ruptures. We evaluated the relationship between AGE content in the diet and accumulation of AGE s in weight‐bearing animal Achilles tendon. Two groups of mice (C57 BL /6Ntac) were fed with either high‐fat diet low in AGE s high‐fat diet ( HFD ) ( n = 14) or normal diet high in AGE s ( ND ) ( n = 11). AGE content in ND was six to 50‐fold higher than HFD . The mice were sacrificed at week 40 and Achilles and tail tendons were carefully excised to compare weight and nonweight‐bearing tendons. The amount of the AGE s carboxymethyllysine ( CML ), methylglyoxal‐derived hydroimidazolone ( MG ‐H1) and carboxyethyllysine ( CEL ) in Achilles and tail tendon was measured using ultraperformance liquid chromatography tandem mass spectrometry ( UPLC ‐ MS / MS ) and pentosidine with high‐pressure liquid chromatography ( HPLC ) with fluorescent detection. AGE s in Achilles tendon were higher than in tail tendon for CML ( P < 0.0001), CEL ( P < 0.0001), MG ‐H1 and pentosidine (for both ND and HFD ) ( P < 0.0001). The AGE ‐rich diet ( ND ) resulted in an increase in CML ( P < 0.0001), MG ‐H1 ( P < 0.001) and pentosidine ( P < 0.0001) but not CEL , in Achilles and tail tendon. This is the first study to provide evidence for AGE accumulation in injury‐prone, weight‐bearing Achilles tendon associated with intake of an AGE ‐rich diet. This indicates that food‐derived AGE s may alter tendon properties and the development of tendon injuries.