z-logo
open-access-imgOpen Access
Regional effects of streptozotocin‐induced diabetes on shortening and calcium transport in epicardial and endocardial myocytes from rat left ventricle
Author(s) -
Smail Manal M. A.,
Qureshi Muhammad A.,
Shmygol Anatoliy,
Oz Murat,
Singh Jaipaul,
Sydorenko Vadym,
Arabi Alya,
Howarth Frank C.,
Al Kury Lina
Publication year - 2016
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.13034
Subject(s) - myocyte , ventricle , medicine , streptozotocin , endocrinology , cardiology , diabetes mellitus
In the heart, the left ventricle pumps blood at higher pressure than the right ventricle. Within the left ventricle, the electromechanical properties of ventricular cardiac myocytes vary transmurally and this may be related to the gradients of stress and strain experienced in vivo across the ventricular wall. Diabetes is also associated with alterations in hemodynamic function. The aim of this study was to investigate shortening and Ca 2+ transport in epicardial ( EPI ) and endocardial ( ENDO ) left ventricular myocytes in the streptozotocin ( STZ )‐induced diabetic rat. Shortening, intracellular Ca 2+ and L‐type Ca 2+ current ( I Ca,L ) were measured by video detection, fura‐2 microfluorimetry, and whole‐cell patch clamp techniques, respectively. Time to peak ( TPK ) shortening was prolonged to similar extents in ENDO and EPI myocytes from STZ ‐treated rats compared to ENDO and EPI myocytes from controls. Time to half ( THALF ) relaxation of shortening was prolonged in ENDO myocytes from STZ ‐treated rats compared to ENDO controls. TPK Ca 2+ transient was prolonged in ENDO myocytes from STZ ‐treated rats compared to ENDO controls. THALF decay of the Ca 2+ transient was prolonged in ENDO myocytes from STZ ‐treated rats compared to ENDO controls. Sarcoplasmic reticulum ( SR ) fractional release of Ca 2+ was reduced in EPI myocytes from STZ ‐treated rats compared to EPI controls. I C a,L activation, inactivation, and recovery from inactivation were not significantly altered in EPI and ENDO myocytes from STZ ‐treated rats or controls. Regional differences in Ca 2+ transport may partly underlie differences in ventricular myocyte shortening across the wall of the healthy and the STZ ‐treated rat left ventricle.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here