Open AccessImpact of external pneumatic compression target inflation pressure on transcriptome‐wide RNA expression in skeletal muscle
Author(s) -
Martin Jeffrey S.,
Kephart Wesley C.,
Haun Cody T.,
McCloskey Anna E.,
Shake Joshua J.,
Mobley Christopher B.,
Goodlett Michael D.,
Kavazis Andreas,
Pascoe David D.,
Zhang Lee,
Roberts Michael D.
Publication year - 2016
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.13029
Subject(s) - transcriptome , compression (physics) , skeletal muscle , inflation (cosmology) , gene expression , rna , microbiology and biotechnology , bioinformatics , biology , anatomy , materials science , gene , physics , biochemistry , composite material , theoretical physics
Next‐generation RNA sequencing was employed to determine the acute and subchronic impact of peristaltic pulse external pneumatic compression ( PEPC ) of different target inflation pressures on global gene expression in human vastus lateralis skeletal muscle biopsy samples. Eighteen ( N = 18) male participants were randomly assigned to one of the three groups: (1) sham ( n = 6), 2) EPC at 30–40 mmHg ( LP ‐ EPC ; n = 6), and 3) EPC at 70–80 mmHg ( MP ‐ EPC ; n = 6). One hour treatment with sham/ EPC occurred for seven consecutive days. Vastus lateralis skeletal muscle biopsies were performed at baseline (before first treatment; PRE ), 1 h following the first treatment ( POST 1), and 24 h following the last (7th) treatment ( POST 2). Changes from PRE in gene expression were analyzed via paired comparisons within each group. Genes were filtered to include only those that had an RPKM ≥ 1.0, a fold‐change of ≥1.5 and a paired t ‐test value of <0.01. For the sham condition, two genes at POST 1 and one gene at POST 2 were significantly altered. For the LP ‐ EPC condition, nine genes were up‐regulated and 0 genes were down‐regulated at POST 1 while 39 genes were up‐regulated and one gene down‐regulated at POST 2. For the MP ‐ EPC condition, two genes were significantly up‐regulated and 21 genes were down‐regulated at POST 1 and 0 genes were altered at POST 2. Both LP ‐ EPC and MP ‐ EPC acutely alter skeletal muscle gene expression, though only LP ‐ EPC appeared to affect gene expression with subchronic application. Moreover, the transcriptome response to EPC demonstrated marked heterogeneity (i.e., genes and directionality) with different target inflation pressures.