Open AccessTransition from physical activity to inactivity increases skeletal muscle miR‐148b content and triggers insulin resistance
Author(s) -
Gastebois Caroline,
Cha Stéphanie,
Rome Sophie,
Durand Christine,
Pelascini Elise,
Jalabert Audrey,
Euthine Vanessa,
Pialoux Vincent,
Blanc Stéphane,
Simon Chantal,
Lefai Etienne
Publication year - 2016
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12902
Subject(s) - skeletal muscle , downregulation and upregulation , insulin resistance , medicine , endocrinology , myogenesis , insulin , chemistry , phosphorylation , biology , microbiology and biotechnology , gene , biochemistry
This study investigated miR‐148b as a potential physiological actor of physical inactivity‐induced effects in skeletal muscle. By using animal and human protocols, we demonstrated that the early phase of transition toward inactivity was associated with an increase in muscle miR‐148b content, which triggered the downregulation of NRAS and ROCK1 target genes. Using human myotubes, we demonstrated that overexpression of miR‐148b decreased NRAS and ROCK1 protein levels, and PKB phosphorylation and glucose uptake in response to insulin. Increase in muscle miR‐148b content might thus participate in the decrease in insulin sensitivity at the whole body level during the transition toward physical inactivity.