
Nanosecond pulsed platelet‐rich plasma (ns PRP ) improves mechanical and electrical cardiac function following myocardial reperfusion injury
Author(s) -
Hargrave Barbara,
Varghese Frency,
Barabutis Nektarios,
Catravas John,
Zemlin Christian
Publication year - 2016
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12710
Subject(s) - medicine , ischemia , cardiology , hsp70 , cardiac function curve , heat shock protein , in vivo , reperfusion injury , hsp27 , oxidative stress , contractility , ventricular fibrillation , heart failure , chemistry , biology , biochemistry , microbiology and biotechnology , gene
Ischemia and reperfusion (I/R) of the heart is associated with biochemical and ionic changes that result in cardiac contractile and electrical dysfunction. In rabbits, platelet‐rich plasma activated using nanosecond pulsed electric fields (ns PRP ) has been shown to improve left ventricular pumping. Here, we demonstrate that ns PRP causes a similar improvement in mouse left ventricular function. We also show that ns PRP injection recovers electrical activity even before reperfusion begins. To uncover the mechanism of ns PRP action, we studied whether the enhanced left ventricular function in ns PRP rabbit and mouse hearts was associated with increased expression of heat‐shock proteins and altered mitochondrial function under conditions of oxidative stress. Mouse hearts underwent 30 min of global ischemia and 1 h of reperfusion in situ. Rabbit hearts underwent 30 min of ischemia in vivo and were reperfused for 14 days. Hearts treated with ns PRP expressed significantly higher levels of Hsp27 and Hsp70 compared to hearts treated with vehicle. Also, pretreatment of cultured H9c2 cells with ns PRP significantly enhanced the “spare respiratory capacity ( SRC )” also referred to as “respiratory reserve capacity” and ATP production in response to the uncoupler FCCP . These results suggest a cardioprotective effect of ns PRP on the ischemic heart during reperfusion.