z-logo
open-access-imgOpen Access
Impact of resistance exercise on ribosome biogenesis is acutely regulated by post‐exercise recovery strategies
Author(s) -
Figueiredo Vandré C.,
Roberts Llion A.,
Markworth James F.,
Barnett Matthew P. G.,
Coombes Jeff S.,
Raastad Truls,
Peake Jonathan M.,
CameronSmith David
Publication year - 2016
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12670
Subject(s) - ribosome biogenesis , muscle hypertrophy , protein biosynthesis , microbiology and biotechnology , ribosomal rna , ribosomal protein s6 , ribosomal protein , eif4e , ribosome , biogenesis , biology , rna , chemistry , medicine , endocrinology , messenger rna , translation (biology) , signal transduction , p70 s6 kinase 1 , biochemistry , pi3k/akt/mtor pathway , gene
Muscle hypertrophy occurs following increased protein synthesis, which requires activation of the ribosomal complex. Additionally, increased translational capacity via elevated ribosomal RNA ( rRNA ) synthesis has also been implicated in resistance training‐induced skeletal muscle hypertrophy. The time course of ribosome biogenesis following resistance exercise ( RE ) and the impact exerted by differing recovery strategies remains unknown. In the present study, the activation of transcriptional regulators, the expression levels of pre‐ rRNA , and mature rRNA components were measured through 48 h after a single‐bout RE . In addition, the effects of either low‐intensity cycling (active recovery, ACT ) or a cold‐water immersion ( CWI ) recovery strategy were compared. Nine male subjects performed two bouts of high‐load RE randomized to be followed by 10 min of either ACT or CWI . Muscle biopsies were collected before RE and at 2, 24, and 48 h after RE . RE increased the phosphorylation of the p38‐ MNK 1‐ eIF 4E axis, an effect only evident with ACT recovery. Downstream, cyclin D1 protein, total eIF 4E, upstream binding factor 1 ( UBF 1), and c‐Myc proteins were all increased only after RE with ACT . This corresponded with elevated abundance of the pre‐ rRNA s (45S, ITS ‐28S, ITS ‐5.8S, and ETS ‐18S) from 24 h after RE with ACT . In conclusion, coordinated upstream signaling and activation of transcriptional factors stimulated pre‐ rRNA expression after RE . CWI , as a recovery strategy, markedly blunted these events, suggesting that suppressed ribosome biogenesis may be one factor contributing to the impaired hypertrophic response observed when CWI is used regularly after exercise.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here