Open AccessImpact of resistance exercise on ribosome biogenesis is acutely regulated by post‐exercise recovery strategies
Author(s) -
Figueiredo Vandré C.,
Roberts Llion A.,
Markworth James F.,
Barnett Matthew P. G.,
Coombes Jeff S.,
Raastad Truls,
Peake Jonathan M.,
CameronSmith David
Publication year - 2016
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12670
Subject(s) - ribosome biogenesis , muscle hypertrophy , protein biosynthesis , microbiology and biotechnology , ribosomal rna , ribosomal protein s6 , ribosomal protein , eif4e , ribosome , biogenesis , biology , rna , chemistry , medicine , endocrinology , messenger rna , translation (biology) , signal transduction , p70 s6 kinase 1 , biochemistry , pi3k/akt/mtor pathway , gene
Muscle hypertrophy occurs following increased protein synthesis, which requires activation of the ribosomal complex. Additionally, increased translational capacity via elevated ribosomal RNA ( rRNA ) synthesis has also been implicated in resistance training‐induced skeletal muscle hypertrophy. The time course of ribosome biogenesis following resistance exercise ( RE ) and the impact exerted by differing recovery strategies remains unknown. In the present study, the activation of transcriptional regulators, the expression levels of pre‐ rRNA , and mature rRNA components were measured through 48 h after a single‐bout RE . In addition, the effects of either low‐intensity cycling (active recovery, ACT ) or a cold‐water immersion ( CWI ) recovery strategy were compared. Nine male subjects performed two bouts of high‐load RE randomized to be followed by 10 min of either ACT or CWI . Muscle biopsies were collected before RE and at 2, 24, and 48 h after RE . RE increased the phosphorylation of the p38‐ MNK 1‐ eIF 4E axis, an effect only evident with ACT recovery. Downstream, cyclin D1 protein, total eIF 4E, upstream binding factor 1 ( UBF 1), and c‐Myc proteins were all increased only after RE with ACT . This corresponded with elevated abundance of the pre‐ rRNA s (45S, ITS ‐28S, ITS ‐5.8S, and ETS ‐18S) from 24 h after RE with ACT . In conclusion, coordinated upstream signaling and activation of transcriptional factors stimulated pre‐ rRNA expression after RE . CWI , as a recovery strategy, markedly blunted these events, suggesting that suppressed ribosome biogenesis may be one factor contributing to the impaired hypertrophic response observed when CWI is used regularly after exercise.