Open AccessAnalysis of metabolic effects of menthol on WFS 1‐deficient mice
Author(s) -
Ehrlich Marite,
Ivask Marilin,
Raasmaja Atso,
Kõks Sulev
Publication year - 2016
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12660
Subject(s) - menthol , trpm8 , basal (medicine) , endocrinology , metabolism , basal metabolic rate , medicine , thermoregulation , chemistry , oral administration , basal body temperature , biology , insulin , transient receptor potential channel , receptor , organic chemistry , trpv1 , hormone , menstrual cycle
In this study, we investigated the physiological regulation of energy metabolism in wild‐type ( WT ) and WFS 1‐deficient (Wfs1 KO ) mice by measuring the effects of menthol treatment on the O 2 consumption, CO 2 production, rectal body temperature, and heat production. The basal metabolism and behavior was different between these genotypes as well as TRP family gene expressions. Wfs1 KO mice had a shorter life span and weighed less than WT mice. The food and water intake of Wfs1 KO mice was lower as well as the body temperature when compared to their WT littermates. Furthermore, Wfs1 KO mice had higher basal O 2 consumption, and CO 2 and heat production than WT mice. In addition, Wfs1 KO mice showed a higher response to menthol administration in comparison to WT mice. The strongest menthol effect was seen on different physiological measures 12 h after oral administration. The highest metabolic response of Wfs1 KO mice was seen at the menthol dose of 10 mg/kg. Menthol increased O 2 consumption, and CO 2 and heat production in Wfs1KO mice when compared to their WT littermates. In addition, the expression of Trpm8 gene was increased. In conclusion, our results show that the Wfs1 KO mice develop a metabolic phenotype characterized with several physiological dysfunctions.