Open AccessExercise‐conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells
Author(s) -
Horsburgh Steven,
Todryk Stephen,
Toms Christopher,
Moran Colin N.,
Ansley Les
Publication year - 2015
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12621
Subject(s) - methyltransferase , peripheral blood mononuclear cell , dnmt3b , epigenetics , dna methylation , dnmt1 , medicine , endocrinology , dna methyltransferase , chemistry , methylation , nuclear dna , andrology , dna , biochemistry , gene expression , in vitro , gene , mitochondrial dna
DNA methylation is modifiable by acute and chronic exercise. DNA methyltransferases ( DNMT ) catalyze this process; however, there is a lack of literature concerning the specific mechanisms by which exercise‐induced modifications occur. Interleukin 6 ( IL ‐6) stimulation of various cell lines has been shown to augment DNMT expression and nuclear translocation, which suggests a possible pathway by which exercise is able to elicit changes in epigenetic enzymes. The present study sought to elucidate the response of the de novo methyltransferases DNMT 3A and DNMT 3B to circulatory factors found in plasma isolated from whole blood before and after 120‐min of treadmill running at an intensity of 60% of individual velocity atV ˙ O 2 max(v V ˙ O 2 max) interspersed with 30‐sec sprints at 90% of v V ˙ O 2 maxevery 10‐min. Peripheral blood mononuclear cells ( PBMC s) isolated from a resting participant were incubated with plasma isolated from exercising participants ( n = 10) or recombinant IL ‐6 ( rIL ‐6), followed by nuclear protein extraction and quantification of DNMT 3A and DNMT 3B concentrations. Nuclear concentrations of DNMT 3B significantly decreased following the experimental protocol ( P = 0.03), with no change observed in DNMT 3A ( P = 0.514).Various concentrations of rIL ‐6 caused an elevation in both DNMT 3A and DNMT 3B nuclear concentration compared with the blank control. The conflicting results between exercising and rIL ‐6 conditions suggests that IL ‐6 does regulate DNMT nuclear transport, however, other plasma mediators may also exert significant influence on the nuclear concentrations of these enzymes.