Open AccessThe response of apoptotic and proteolytic systems to repeated heat stress in atrophied rat skeletal muscle
Author(s) -
Yoshihara Toshinori,
Sugiura Takao,
Yamamoto Yuki,
Shibaguchi Tsubasa,
Kakigi Ryo,
Naito Hisashi
Publication year - 2015
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12597
Subject(s) - soleus muscle , atrophy , muscle atrophy , skeletal muscle , hindlimb , heat stress , medicine , apoptosis , endocrinology , chemistry , biology , biochemistry , zoology
We examined the effect of repeated heat stress on muscle atrophy, and apoptotic and proteolytic regulation in unloaded rat slow‐ and fast‐type skeletal muscles. Forty male Wistar rats (11 week‐old) were divided into control ( CT ), hindlimb unweighting ( HU ), intermittent weight‐bearing during HU ( HU + IWB ), and intermittent weight‐bearing with heat stress during HU (41–41.5°C for 30 min; HU + IWB + HS ) groups. The HU + IWB + HS and HU + IWB groups were released from unloading for 1 h every second day, during which the HU + IWB + HS group underwent the heating. Our results revealed that repeated bouts of heat stress resulted in protection against disuse muscle atrophy in both soleus and plantaris muscles. This heat stress–induced protection against disuse‐induced muscular atrophy may be partially due to reduced apoptotic activation in both muscles, and decreased ubiquitination in only the soleus muscle. We concluded that repeated heat stress attenuated skeletal muscle atrophy via suppressing apoptosis but the response to proteolytic systems depend on the muscle phenotype.