Open AccessUnique gene program of rat small resistance mesenteric arteries as revealed by deep RNA sequencing
Author(s) -
Reho John J.,
Shetty Amol,
Dippold Rachael P.,
Mahurkar Anup,
Fisher Steven A.
Publication year - 2015
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12450
Subject(s) - biology , myosin , mesenteric arteries , gene expression , microbiology and biotechnology , skeletal muscle , medicine , endocrinology , gene , artery , biochemistry
Deep sequencing of RNA samples from rat small mesenteric arteries ( MA ) and aorta ( AO ) identified common and unique features of their gene programs. ~5% of mRNA s were quantitatively differentially expressed in MA versus AO . Unique transcriptional control in MA smooth muscle is suggested by the selective or enriched expression of transcription factors Nkx2‐3, HAND 2, and Tcf21 (Capsulin). Enrichment in AO of PPAR transcription factors and their target genes of mitochondrial function, lipid metabolism, and oxidative phosphorylation is consistent with slow (oxidative) tonic smooth muscle. In contrast MA was enriched in contractile and calcium channel mRNA s suggestive of components of fast (glycolytic) phasic smooth muscle. Myosin phosphatase regulatory subunit paralogs Mypt1 and p85 were expressed at similar levels, while smooth muscle MLCK was the only such kinase expressed, suggesting functional redundancy of the former but not the latter in accordance with mouse knockout studies. With regard to vaso‐regulatory signals, purinergic receptors P2rx1 and P2rx5 were reciprocally expressed in MA versus AO , while the olfactory receptor Olr59 was enriched in MA . Alox15, which generates the EDHF HPETE , was enriched in MA while eNOS was equally expressed, consistent with the greater role of EDHF in the smaller arteries. mRNA s that were not expressed at a level consistent with impugned function include skeletal myogenic factors, IKK 2, nonmuscle myosin, and Gnb3. This screening analysis of gene expression in the small mesenteric resistance arteries suggests testable hypotheses regarding unique aspects of small artery function in the regional control of blood flow.