Open AccessEssential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets
Author(s) -
Hiasa Miki,
Togawa Natsuko,
Miyaji Takaaki,
Omote Hiroshi,
Yamamoto Akitsugu,
Moriyama Yoshinori
Publication year - 2014
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12034
Subject(s) - nucleotide , platelet , vesicular transport protein , transporter , adenine nucleotide , microbiology and biotechnology , biochemistry , biology , chemistry , membrane , vesicle , gene , immunology
Nucleotides are stored in the dense granules of platelets. The release of nucleotides triggers one of the first steps in a series of cascades responsible for blood coagulation. However, the mechanism of how the nucleotides are accumulated in the granules is still far less understood. The transporter protein responsible for storage of nucleotides in the neuroendocrine cells has been identified and characterized. We hypothesized that the vesicular nucleotide transporter ( VNUT ) is also involved in the vesicular storage of nucleotides in platelets. In this article, we present three lines of evidence that VNUT is responsible for the vesicular storage of nucleotides in platelets and that vesicular ATP transport is crucial for platelet function, detection and characterization of VNUT activity in platelets isolated from healthy humans and MEG ‐01 cells, RNA interference experiments on MEG ‐01 cells, and studies on nucleotide transport and release with a selective inhibitor.