Open AccessCardioprotective effects of dietary lipids evident in the time‐dependent alterations of cardiac function and gene expression following myocardial infarction
Author(s) -
Berthiaume Jessica M.,
Azam Salaman M.,
Hoit Brian D.,
Chandler Margaret P.
Publication year - 2014
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12019
Subject(s) - cardioprotection , cardiac function curve , myocardial infarction , lipid metabolism , medicine , ejection fraction , endocrinology , ventricular remodeling , heart failure , protein kinase b , metabolism , beta oxidation , hemodynamics , cardiology , apoptosis , biology , biochemistry
We have previously shown that prolonged high–saturated fat feeding ( SAT ) for 8 weeks after myocardial infarction ( MI ) improves ventricular function and prevents the metabolic remodeling commonly observed in heart failure. The current study was designed to delineate the interplay between markers of energy metabolism and indices of cardiac remodeling with 2 and 4 weeks of post‐ MI SAT in male W istar rats. By 2 weeks, less remodeling was noted in MI ‐ SAT evidenced by diminished chamber dilation and greater ejection fraction assessed by echocardiography and hemodynamic measures. In addition, gene expression of energy metabolism targets involved in FA uptake, oxidation, and glucose oxidation regulation was increased in MI ‐ SAT with respect to MI alone, although no change in PDH phosphorylation was observed. The regulatory kinase, phosphoinositide 3 kinase ( P i3k ), was strongly induced by 2 weeks in the MI ‐ SAT group, although AKT protein content (a primary downstream target of PI 3 K that affects metabolism) was decreased by both MI and SAT alone, indicating early involvement of cellular signaling pathways in lipid‐mediated cardioprotection. Our results demonstrate that cardioprotection occurs acutely with SAT following MI , with improvement in indices of both cardiac function and fatty acid oxidation, suggesting a mechanistic role for energy metabolism in the beneficial effects of high dietary fat following cardiac injury.