Open AccessProtective effect and localization by optical imaging of human renal CD 133 + progenitor cells in an acute kidney injury model
Author(s) -
Grange Cristina,
Moggio Aldo,
Tapparo Marta,
Porta Stefano,
Camussi Giovanni,
Bussolati Benedetta
Publication year - 2014
Publication title -
physiological reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 39
ISSN - 2051-817X
DOI - 10.14814/phy2.12009
Subject(s) - renal stem cell , progenitor cell , mesenchymal stem cell , kidney , stem cell , medicine , regeneration (biology) , pathology , acute kidney injury , stem cell transplantation for articular cartilage repair , bone marrow , endothelial stem cell , regenerative medicine , cancer research , microbiology and biotechnology , adult stem cell , biology , in vitro , biochemistry
Recent approaches of regenerative medicine can offer a therapeutic option for patients undergoing acute kidney injury. In particular, mesenchymal stem cells were shown to ameliorate renal function and recovery after acute damage. We here evaluated the protective effect and localization of CD 133 + renal progenitors from the human inner medulla in a model of glycerol‐induced acute tubular damage and we compared the results with those obtained with bone marrow‐derived mesenchymal stem cells. We found that CD 133 + progenitor cells promoted the recovery of renal function, preventing tubular cell necrosis and stimulating resident cell proliferation and survival, similar to mesenchymal stem cells. In addition, by optical imaging analysis, CD 133 + progenitor cells accumulated within the renal tissue, and a reduced entrapment in lung, spleen, and liver was observed. Mesenchymal stem cells were detectable at similar levels in the renal tissue, but a higher signal was present in extrarenal organs. Both cell types produced several cytokines/growth factors, suggesting that a combination of different mediators is involved in their biological action. These results indicate that human CD 133 + progenitor cells are renotropic and able to improve renal regeneration in acute kidney injury.