Open AccessProduction of xylanase by Fusarium oxysporum using agro-industrial waste
Author(s) -
Lunara Thaís Alves de Bastos,
Ryhára Dias Batista,
Alanna Cristinne Martins Lima,
Iara Leandro Dos Santos,
Fabrício Coutinho de Paula-Elias,
Cláudia Cristina Auler do Amaral Santos,
Alex Fernando de Almeida
Publication year - 2021
Publication title -
scientia plena
Language(s) - English
Resource type - Journals
ISSN - 1808-2793
DOI - 10.14808/sci.plena.2021.081511
Subject(s) - corncob , xylanase , husk , fusarium oxysporum , bagasse , straw , food science , microbiology and biotechnology , pulp and paper industry , chemistry , mathematics , agronomy , biology , botany , raw material , biochemistry , organic chemistry , enzyme , engineering
This study aimed to optimize the production of xylanase, under submerged conditions using Fusarium oxysporum. Firstly, it was carried out the selection of the best source of carbon from agricultural waste, such as soybean husk, cassava peel, pineapple crown, corn straw, rice husk, bacaba, barley bagasse and, corncob. They were milled and sieved with a maximum granulation of 1 mm. The cultures were carried out by 132 hours 30 °C and 180 rpm. Among the agroindustrial residues, corn straw was the one that stood out with a greater enzymatic activity of 12.6 U/mL. In the second step, it was used Plackett-Burman design to screen the nutrients sources important to xylanase production. Thus, independent variables significant were urea and MgSO4. These variables selected by Plackett-Burman were then used in a Central Composite Rotational Design present activity of 26.6 U/mL and the predicted was 34.5 U/mL. So, the xylanase production by F. oxysporum can be optimize using corn straw, a low-cost waste found in large quantities.