Open AccessAvaliação do extrato metanólico de urucum (Bixa orellana L.) em associação com azul de metileno para dupla coloração em histologia vegetal
Author(s) -
Davi Ângelo Costa Leal,
Mônica da Costa Serra,
Matheus Brandão Campos,
Hiran Reis Sousa,
Wermerson Assunção Barroso,
Crisálida Machado Vilanova
Publication year - 2018
Publication title -
scientia plena
Language(s) - English
Resource type - Journals
ISSN - 1808-2793
DOI - 10.14808/sci.plena.2018.081201
Subject(s) - methylene blue , maceration (sewage) , orange (colour) , distilled water , staining , chemistry , sodium hypochlorite , botany , traditional medicine , food science , nuclear chemistry , chromatography , biology , biochemistry , materials science , organic chemistry , medicine , photocatalysis , composite material , genetics , catalysis
The annatto is a yellow-orange dye extracted from the pulp of Bixa orellana L. seeds, a tree native of tropical forests. The composition of this dye consists basically of carotenoids, such as bixin, norbixin and nobixate. Bixin, a highest amount compound, has a liposoluble characteristic that facilitates its interaction with cell membranes. The present study evaluated the dye association viability in vegetable histology of Bixa orellana L., Morinda citrifolia L. and Petiveria alliacea L. leaves. The annatto dye was obtained by extraction in methanol (1:6 w/v) by maceration of Bixa orellana seeds. The analyzes were carried out in cross sections of the vegetal samples collected in the “Berta Lange Morretes” Medical Garden of Federal University of Maranhão (UFMA). After clarification with sodium hypochlorite, the staining was performed in an average immersion time of 10 seconds, for both the urucum + methylene blue association in the 2:1 (A1) and 1:1 (A2) concentrations as well as for the blue methylene 1% and annatto dye 2%. All sections were mounted between blade and coverslip with distilled water, and visualized under an optical microscope. The A1 allowed a better morphological visualization of the anatomical structures due to the acidic characteristic of bixin which reacts with basic pH components of plant, and the alkaline characteristic of methylene blue that reacts with structures of acidic nature. This association represents a staining technique that can contribute alternatively as plant histological dyes.