Open AccessDynamics of TNF-α and INF-γ content in blood serum in connection with the marker profile of patients with acute hepatitis B at different degrees of severity of the disease
Author(s) -
A. B. Khelemendyk
Publication year - 2022
Publication title -
aktualʹnì pitannâ farmacevtičnoï ì medičnoï nauki ta praktiki
Language(s) - English
Resource type - Journals
eISSN - 2409-2932
pISSN - 2306-8094
DOI - 10.14739/2409-2932.2022.1.251211
Subject(s) - medicine , hbsag , gastroenterology , seroconversion , hbeag , liver disease , immunology , hepatitis b virus , antibody , virus
The aim – is to analyze the dynamics of the content of TNF-α and INF-γ in the serum in connection with the marker profile of patients with acute hepatitis B at different degrees of severity of the disease.Materials and methods. 32 patients with AHB were examined in the dynamics of the disease with the determination of HBsAg, HBeAg, anti-HBs and anti-HBe, the content of TNF-α and IFN-γ by ELISA. Statistical processing was performed in Statistica 13 for Windows (StatSoft Inc., No. JPZ804I382130ARCN10-J).Results. Patients with severe (40.6 %) and moderate (37.5 %) courses of the disease predominate among patients with AHB. Severe course of AHB, compared with the moderate course, was characterized by higher levels of hyperbilirubinemia (P 0.05) course of the disease. Regardless of the severity of AHB, the content of TNF-α was higher (P < 0.05) than in healthy individuals and highest in the severe course of the disease (P < 0.05). The content of INF-γ was higher than in healthy individuals (P < 0.05), regardless of the severity of the disease. TNF-α correlated with the severity of AHB (r = 0.57, P < 0.05), the level of total bilirubin (r = 0.65, P < 0.05), INR (r = 0.42, P < 0.05) and PI (r = -0.48, P < 0.05) at hospitalization.Conclusions. Patients with severe (40.6 %) and moderate (37.5 %) courses of the disease predominate among the hospitalized patients with AHB. At hospitalization, the content of TNF-α is highest in the severe course of the disease (P < 0.05). The content of INF-γ is higher than in healthy individuals (P < 0.05), regardless of the severity of the course of AHB. At hospitalization, all patients were positive for HBsAg and IgM anti-HBcorAg, the vast majority of patients for HBeAg. Positive HBsAg was more often (P < 0.05) in patients with a moderate course of AHB during discharge from the hospital. HBeAg was preserved in 28.6 % of patients with the mild course. Chronic HBV infection was observed in 3.6 % of patients with the mild course.