Open AccessAntibacterial and antioxidant activities of endophytic fungi and nettle (Urtica dioica L.) leaves as their host
Author(s) -
Djouza Salmi,
Catherine Riou,
Mohammad Issawi,
Yacine Titouche,
Veronica Ambrosini,
Noria Smail-Saadoun,
Hocine Abbaci,
Karim Houali
Publication year - 2021
Publication title -
cellular and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.371
H-Index - 71
eISSN - 1165-158X
pISSN - 0145-5680
DOI - 10.14715/cmb/2021.67.3.33
Subject(s) - urtica dioica , dpph , plant use of endophytic fungi in defense , antimicrobial , endophyte , biology , traditional medicine , antibacterial activity , botany , antioxidant , superoxide dismutase , bacteria , microbiology and biotechnology , biochemistry , medicine , genetics
Nettle (Urtica dioica L), as a plant rich in biologically active compounds, is one of the most important plants used in herbal medicine. Studies have shown that this plant has antioxidant, antiplatelet, hypoglycemic and hypocholesterolemia effects. In this study, we characterized three Alternaria endophytic fungi isolated from their host U. dioica. We hypothesized that these endophytic fungi can produce new bioactive metabolites, which may possess the bioactive property with potential application in the medical and pharmaceutical industries. The antibacterial activity was evaluated against reference and isolated strains, including Methicillin-Resistant Staphylococcus aureus. A wide range of antimicrobial activities similar to those measured in nettle leaves was detected especially for Alternaria sorghi. Furthermore, the highest antioxidant activity detected with DPPH free radical scavenging was measured for A. sorghi and nettle leaves ethyl acetate extracts. In addition, whereas catalase activity was similar in the three isolated fungi and nettle leaves, total thiol content and superoxide dismutase activity were significantly higher in leaves. A. sorghi showed the best activities compared to other isolated fungi. The characterization and further production of bioactive compounds produced by this endophyte should be investigated to fight bacteria and especially those that develop drug multi-resistance.