Open AccessRadiation-induced graft polymerization of elastin onto polyvinylpyrrolidone as a possible wound dressing
Author(s) -
María Luisa Del Prado-Audelo,
Francisco J. Gómez-Zaldivar,
Mario Alberto Pérez-Díaz,
Roberto Sánchez-Sánchez,
Maykel GonzálezTorres,
Manuel GonzálezDel Carmen,
Gabriela FigueroaGonzález,
Javad SharifiRad,
Octavio Daniel ReyesHernández,
Hernán Cortés,
Gerardo Leyva-Gómez
Publication year - 2021
Publication title -
cellular and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.371
H-Index - 71
eISSN - 1165-158X
pISSN - 0145-5680
DOI - 10.14715/cmb/2021.67.1.10
Subject(s) - polyvinylpyrrolidone , self healing hydrogels , thermogravimetric analysis , differential scanning calorimetry , biocompatibility , fourier transform infrared spectroscopy , swelling , viability assay , wound healing , materials science , chemical engineering , polymerization , polymer chemistry , chemistry , nuclear chemistry , biomedical engineering , polymer , composite material , surgery , cell , biochemistry , medicine , physics , engineering , thermodynamics , metallurgy
The purpose of our study was to obtain new wound dressings in the form of hydrogels that promote wound healing taking advantage of the broad activities of elastin (ELT) in physiological processes. The hydrogel of ELT and polyvinylpyrrolidone (PVP; ELT–PVP) was obtained by cross-linking induced by gamma irradiation at a dose of 25 kGy. The physicochemical changes attributed to cross-linking were analyzed through scanning electron microscopy (SEM), infrared spectroscopy analysis with Fourier transform (FTIR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Furthermore, we performed a rheological study to determine the possible changes in the fluidic macroscopic properties produced by the cross-linking method. Finally, we accomplished viability and proliferation analyses of human dermal fibroblasts in the presence of the hydrogel to evaluate its biological characteristics. The hydrogel exhibited a porous morphology, showing interconnected porous with an average pore size of 16 ± 8.42 µm. The analysis of FTIR, DSC, and TGA revealed changes in the chemical structure of the ELT–PVP hydrogel after the irradiation process. Also, the hydrogel exhibited a rheological behavior of a pseudoplastic and thixotropic fluid. The hydrogel was biocompatible, demonstrating high cell viability, whereas ELT presented low biocompatibility at high concentrations. In summary, the hydrogel obtained by gamma irradiation revealed the appropriate morphology to be applied as a wound dressing. Interestingly, the hydrogel exhibited a higher percentage of cell viability compared with ELT, suggesting that the cross-linking of ELT with PVP is a suitable strategy for biological applications of ELT without generating cellular damage.