Open AccessDown-regulation of miR-556-3p inhibits hemangioma cell proliferation and promotes apoptosis by targeting VEGFC
Author(s) -
Jia Wen,
Changhong Liang,
Fang Gao,
Mei Yang,
Yan Liu,
Bo Wang
Publication year - 2020
Publication title -
cellular and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.371
H-Index - 71
eISSN - 1165-158X
pISSN - 0145-5680
DOI - 10.14715/cmb/2020.66.5.34
Subject(s) - apoptosis , transfection , microrna , cell growth , annexin , cancer research , mtt assay , vascular endothelial growth factor c , microbiology and biotechnology , chemistry , vascular endothelial growth factor a , biology , cell culture , vascular endothelial growth factor , gene , vegf receptors , genetics , biochemistry
The purpose of this study was to determine the effect of microRNA (miR)-556-3p on cell proliferation and apoptosis of hemangioma-derived endothelial cells (HemECs). qRT-PCR was used to compare the expressions of miR-556-3p in HemECs and normal cells. The target gene was identified using dual-luciferase reporter assay. Cell proliferation was measured with MTT assay, while western blotting was used to assay VEGFC expression levels. Apoptosis was assayed with FITC Annexin V Apoptosis assay kit. miR-556-3p was overexpressed in HemECs. Transfection with miR-556-3p inhibitor resulted in decreased proliferation of HemECs (p<0.05). The expression of the target gene of miR-556-3pi.e. VEGFC was upregulated when miR-556-3p inhibitor was transfected. The transfection also resulted in increased apoptosis. In HemECs, miR-556-3p is overexpressed and VEGFC expression is low. Thus, miR-556-3p or VEGFC might be potential targets for treatment of angiosarcoma.