Open AccessVisfatin increases miR-21 to promote migration in HCC
Author(s) -
NingLin Liang,
Yongjun Chen,
Yang Li,
Suyu He,
Tianyu Li
Publication year - 2018
Publication title -
cellular and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.371
H-Index - 71
eISSN - 1165-158X
pISSN - 0145-5680
DOI - 10.14715/cmb/2018.64.6.9
Subject(s) - downregulation and upregulation , hepatocellular carcinoma , medicine , metastasis , malignancy , pathological , cell migration , transfection , cancer research , cell , endocrinology , cell culture , cancer , biology , gene , biochemistry , genetics
Hepatocellular carcinoma (HCC) is a common human malignancy. In this study, we aimed to investigate the serum levels of visfatin and miR-21 in HCC patients, to analysis the relationship between the pathological features and the plasma level of visfatin or miR-21, and to explore the roles of visfatin and miR-21 in migration of HCC cells. Our results showed that the serum levels of visfatin and miR-21 were significant higher in HCC patients than healthy subjects. The diagnostic sensitivity of serum visfatin was 82.5% and the specificity was 65.0%. The serum visfatin was significantly associated with the histology and metastasis. Visfatin induced miR-21 expression and cell migration in HepG2 cells. Transfection of miR-21 inhibitor suppressed the visfatin-induced migration in HCC cells. These results suggested that visfatin induced HCC cell migration via upregulation of miR-21, which provides a novel basis for the diagnosis of HCC.